TOPIC A:
Bench approaches to pain (max 8 participants)

– Nervous system manipulation by stereotaxic surgery
How to achieve blind high precision approach of particular neural areas for focal stimulation/blockade, focal lesioning, drug delivery or detection of event-related changes in neurotransmission (microdialysis).
– Methods for quantifying pain in animal models
How to measure experimentally elicited or spontaneous pain in experimental animals
– Nervous system electrophysiological recording in awake behaving animals

 

TOPIC B:
Establishment of intervertebral disc organ cultures (max 8 participants)

– Isolation of intervertebral discs from bovine tails
– Organ cultures of healthy and degenerated intervertebral discs
– Evaluation of cartilaginous ECM of bovine intervertebral disc

 

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